Network Analysis

CETSA responses are not independent. Proteins often show coordinated stability changes due to shared pathways or complexes.

Co-stabilization network

The pipeline constructs a network where:

• Nodes represent proteins
• Edges represent correlation of dose–response profiles

Steps

• Compute correlation matrix across proteins
• Apply threshold to define edges
• Build graph representation
• Detect modules using community detection

Modules

Modules represent groups of proteins with similar response patterns.

These often correspond to:

• protein complexes
• metabolic pathways
• co-regulated systems

Interpretation

• Dense modules suggest coordinated biological processes
• Edge weights reflect similarity in response behavior
• Network structure reveals system-level organization

This layer converts CETSA data into a functional interaction landscape.